Specific detection of target <i>C. difficile</i> strains by qPCR with newly developed oligonucleotide sets.

ana, not applicable. bThe reactivity of qPCR for the target bacteria with each primers-probe set was investigated by using DNA extracts corresponding to 105 cells per reaction from each pure culture of the listed strains. Reactivity was judged by using the criteria described in the Materials and Methods. In addition, negative PCR results were obtained for the following bacterial strains, representing the major intestinal bacteria: Blautia productus JCM 1471T, Faecalibacterium prausnitzii ATCC 27768T, Bacteroides vulgatus ATCC 8482T, Bacteroides ovatus JCM 5824T, Fusobacterium varium ATCC 8501T, Collinsella aerofaciens ATCC 25986T, Prevotella melaninogenica ATCC 25845T, Veillonella parvula GIFU 7884T, Bifidobacterium longum ATCC 15707T, Bifidobacterium adolescentis ATCC 15703T, Bifidobacterium catenulatum ATCC 27539T, Lactobacillus gasseri DSM 20243T. Specific detection of target C. difficile strains by qPCR with newly developed oligonucleotide sets.