Droplet-based single-cell pairing for high-throughput interaction mapping of antigen-receptor combinations

Mapping the interaction potential of different variant combinations of viral antigens and human cell receptors and understanding how viral antigen mutations interact with human genetic polymorphisms are critical for predicting infection susceptibility and informing precision public health strategies. Here, we develop a droplet-based single-cell pairing and library-on-library interaction screening (SPLIS) system for high-throughput profiling of the syncytium-formation landscapes of various spike-angiotensin-converting enzyme 2 (ACE2) variant combinations. This system uses combined droplet sorting and merging to deterministically encapsulate one antigen-presenting sender cell and one receptor-expressing receiver cell into each drop, followed by selection and sequencing of the fused DNA readouts to characterize the syncytium-formation potential of each combination. We applied SPLIS to characterize both fusion-enhancing and -inhibiting variant pairs, comprehensively profiling how ACE2 singl..., , # Data from: Droplet-based single-cell pairing for high-throughput interaction mapping of antigen-receptor combinations

Dataset DOI: [10.5061/dryad.6djh9w1fw](https://doi.org/10.5061/dryad.6djh9w1fw)

## Description of the data and file structure

We have submitted our raw data for **Critical evaluation of droplet manipulations** with (**Fig._3A.opju, Fig._3B.opju** and **Fig._3E.opju**). We have also added raw data for **Validation of droplet synchronization on nonuniformly sized droplets** (**Fig._S3A.opju** and **Fig._S3B.opju**), as well as raw data for **Encounter frequency at different sizes of droplets** (**Fig._S4.opju**).

### Files and variables

**File:** Fig._3A.opju

**Description:** The file contains 6 tables for Fig. 3A.

* **The red square** contains tested and fitted plots showing the droplet diameter (μm) and generation frequency when the water flow rate is at 100 μL/h.
* **The green square** contains tested and fitted plots showing the droplet diameter (μm) and gener..., ,