five

poly A+ RNA sequencing of cell cycle-synchronized RNA from U2OS cells

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NIAID Data Ecosystem2026-03-12 收录
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https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE143275
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We performed paired-end poly A+ RNA sequencing using total RNA isolated from U2OS cells that are synchronised into G1, G1/S, S, G2 and M phase of cell cycle. M-phase cells were collected using Nocadozole treatment. G1 cell are isolated using Nocadozole arrest followed by release. G1/S, S and G2 cells were collected by performing double-thymidine block and release.
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2020-11-02
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