Supporting Information.

Strains and bacteriophages used in this study (Table A). Plasmids and primer pairs used in this study (Table B). Bacterial survival rate as a function of time under several temperature conditions (Table C). Fig A. Fluorescence emission upon infection with engineered HK620 phages. Upper panels, bacterial growth and fluorescence profile obtained from non infected E. coli TD2158 alone (red), infected with WT HK620 (orange) or recombinant phages HK620::PbolA-gfp (purple), HK620::PrplU-gfp (green), HK620::PrrnB-gfp (blue) were compared. Top panels represent growth curves (top left panel) and culture fluorescence (right panel) obtained using a microplate reader. Bacterial growth, lytic and lysogenic growth phases are indicated by 1, 2 and 3, respectively. Fluorescence intensity was determined using the equation: (sample fluorescence – medium autofluorescence) / OD600. Bottom panel, engineered phages conservation was studied over 1 year by measuring phages titers and post-infection fluorescence intensities. Column histograms represent fluorescence intensities obtained after 1 hour incubation at 37°C of E. coli TD2158 infected with WT HK620 (orange) or recombinant phages HK620::PrplU-gfp (green), HK620::PrrnB-gfp (blue). Fluorescence intensity was obtained using a microplate reader and determined with the equation: (sample fluorescence – medium autofluorescence) / OD600. Stability of phages during several weeks is represented by titration curves of WT and recombinant HK620 phages.
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