Transcriptome analysis and 5'-end mapping of total RNA from Rhodobacter sphaeroides wild type and RNase E (rne) mutant strain, grown under aerobic, microaerobic or phototrophic conditions

The conserved endoribonuclease RNase E is essential in Rhodobacter sphaeroides and acts as global regulator of the transcriptome. By comparison of an RNase E mutant (showing reduced enzyme activity) with the Rhodobacter sphaeroides wild type, both grown under three different growth conditions, we analysed the impact of RNase E on the adaption of Rhodobacter sphaeroides to different growth conditions. Overall design: Comparative gene expression profiling analysis of RNAseq data of independent biological triplicates of Rhodobacter sphaeroides wild type and RNase E (rne) mutant strain under three different growth conditions (aerobic, microaerobic or phototrophic).