Assay reproducibility of <i>Coccidioides</i> duplex real-time PCR.

(A) Inter-assay reproducibility: DNA of varying concentrations from three isolates each of C. posadasii and C. immitis were tested in duplicate on 3 separate days. The Ct values obtained were consistent with % CV of <5%, which confirmed an excellent inter-assay reproducibility, (B) Intra-assay reproducibility: DNA samples from three isolates each of C. immitis and C. posadasii with varying concentrations were tested in triplicate on the same day on same plate in one run. The Ct values obtained were consistent with % CV of <5%, which confirmed an excellent intra-assay reproducibility. (XLSX)