RNA sequencing of wild-type S. cerevisiae (BY4741) thermo-pulsed with optimal ( 30 °C- 30 °C) or elevated ( 30 °C- 37 °C) temperatures

We conducted paired-end RNA sequencing on Saccharomyces cerevisiae (BY4741) to study the effects of different thermal pulsing conditions. The cells were divided into two groups: thermal pulsing (TP) and steady-state (SS) conditions. In the TP condition, cells were initially incubated at 30°C in YPD broth for 15 minutes, followed by 15 minutes at 37°C, constituting a single cycle of thermal pulsing. In the SS condition, cells were maintained at 30°C for both 15-minute intervals. This process was repeated for twenty-five cycles to produce both thermally pulsed cells and steady-state cells.