Data from: Photothermal-assisted antibacterial application of GO-Ag nanocomposites against clinical isolated MDR E. coli

In the field of public health, treatment of multidrug-resistant (MDR)
bacterial infection is a great challenge. Herein, we provide a solution to
this problem with the use of graphene oxide-silver (GO-Ag) nanocomposites
as anti-bacterial agent. Following established protocols, silver
nanoparticles were grown on graphene oxide sheets. Then, a series of
in-vitro studies were conducted to validate the antibacterial efficiency
of the GO-Ag nanocomposites against clinical MDR Escherichia coli (E.
coli) strains. GO-Ag nanocomposites showed the highest antibacterial
efficiency among tested antimicrobials (graphene oxide, silver
nanoparticles, GO-Ag), and synergetic antibacterial effect was observed in
GO-Ag nanocomposites treated group. 14.0 µg mL-1 GO-Ag treatment could
greatly inhibit bacteria growth, remained bacteria viabilities were 4.4%
and 4.1% for MDR-1 and MDR-2 E. coli bacteria respectively. In addition,
with assistance of photothermal effect, effective sterilization could be
achieved using GO-Ag nanocomposites as low as 7.0 µg mL-1. Fluorescence
imaging and morphology characterization uncovered that bacteria integrity
was disrupted after GO-Ag nanocomposites treatment. Cytotoxicity results
of GO-Ag using human derived cell lines (HEK 293T, Hep G2) suggested more
than 80% viability remained at 7.0 µg mL-1. All the results proved that
GO-Ag nanocomposites are efficient antibacterial agent against multi-drug
resistant E. coli.