Wild boar gut microbiota metagenome

Wild boar gut microbiota 16S rRNA amplicon sequencing.Amplicon metagenome analysis to describe gut microbial communities from feces samples taken from the end of the intestine from 88 animals hunted in 16 hunting estates. Raw sequences from 16S rRNA amplicon sequencing (hiper-variable V4 region). We studied wild boar populations in hunting estates in Spain that ranged in size between 750 and 3000 Ha. The typical habitat of wild boar populations included mountain ranges and flat terrain, covered by Mediterranean vegetation. We took morphological measurements and hair and feces samples from adult wild boar harvested in 16 populations in the south-west of Spain during 2019 and 2020. All individuals came from legal hunt during the hunting season (from 15th October to 15th February). Ethical issues were revised and approved by UIRCP (Wildlife Research Unit), University of Cordoba. Age class was established according to eruption and tooth wear. All boar faeces were immediately preserved in lysis buffer (40 mM EDTA; 50 mM Tris pH 8.3; 0.75 M sucrose) after collection and further frozen (-20 C degrees) until DNA extraction.