Gene expression profile at single embryo level of 0-3h old Drosophila embryos

We used single-embryo sequencing to characterize early zygotic gene expression in Drosophila. The data provide the first continuous timeline of transcript levels during early development (= 3 hours) in Drosophila melanogaster. Overall design: RNA from single Drosophila embryos was isolated and subjected to a modified CelSeq2 single-cell RNA-sequencing protocol. Pseudo-temporal analysis of the single embryo transcriptomes provides a high-resolution, time-sensitive picture of transcriptional events during early embryonic development.