Master Regulators of oncogenic KRAS response in Pancreatic Cancer. Mus musculus

We derived a transcriptional signature of oncogenic KRAS by using the KF508 murine pancreatic ductal cell line with an inducible Lox-Stop-Lox (LSL) cassette in front of the KRASG12D oncogene to regulate transcription. This dataset allowed us to study the differential expression profile after oncogenic KRAS induction in mouse. Overall design: Total RNA was extracted from six sets of mock and cre treated ductal cells using Qiagen RNeasy kit. The RNA was labelled and hybridized. This experiment was carried out on 2 Illumina Mousev2 BeadChips using 24 arrays (1 samples per Bead-Chip).