Human HEK293 Gemin5 clinical variants LC-MSMS

Identification of cellular proteins interacting with Gemin5 variants found in patients with neurological disorders. P85 C-terminal fragment of Gemin5 has been used to purify the protein complexes associated by Tandem Affinity Purification. The study has been performed using two biological replicates of each bait protein: p85-WT, the dimerization mutant p85-A951E, and the clinical variants p85-R1016C, p85-D1019E and p85-L1367P.