Silencing of dynamin II decreases apoE secretion.
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HMDM were incubated with 150 nM non-silencing or dyn II siRNA oligo’s for 48 h, followed by a further incubation for 72 h in RPMI containing 10% LPDS and 50 µg/mL AcLDL. After loading apoE secretion was determined over a 2 h period in 0.1% BSA in RPMI. Cellular dynamin II levels (A) and secreted apoE (B) were determined by Western blotting and ELISA, respectively. Data are mean ± SD from triplicate cultures representative of at least 2 individual donors. *Pv Ctrl.
创建时间：
2016-02-23
