Zea mays Transcriptome or Gene expression

ECB larvae remain damaging pests of conventiational maize production. To better understand the physiological and molecular changes occuring in stem tissue during insect attack we explored the biochemical and transcriptional profiles in these tissues. We used microarrays to detail the transcriptional changes associated with ECB attack and considered these in the context of biochemical markers of plant defense and total protein levels. Overall design: Maize internode tissue was selected from untreated control plants and those which 5th instar ECB larvae had bored into for 48 h for RNA extraction and hybridization on Affymetrix microarrays.

欧洲玉米螟幼虫是传统玉米生产的危害性害虫。为深入理解昆虫侵袭期间茎组织所发生的生理和分子变化，我们探究了这些组织中的生化及转录谱。我们运用微阵列技术详细解析了与欧洲玉米螟侵袭相关的转录变化，并在植物防御生化标志物及总蛋白水平的基础上进行综合考量。整体设计：选取未经处理的对照组玉米植株及第五龄期欧洲玉米螟幼虫侵袭48小时后的玉米节间组织，用于RNA提取及Affymetrix微阵列杂交。