2-5A-Mediated mRNA Decay and Transcription Act in Concert to Reprogram Protein Synthesis during dsRNA Response

RNA degradation by RNase L during 2-5A-mediated decay (2-5AMD) is a conserved mammalian stress response to viral and endogenous double-stranded RNA (dsRNA). To understand this mechanism we examined 2-5AMD in human cells by spike-in RNA-seq. Overall design: Poly-IC transfection for 0-4 h followed by poly-A pulldown and RNAseq