FvE J Does Not Induce a Type I IFN Response

(A) Mock- or lentivirally transduced Vero cells were challenged with JEV at a MOI of 10, and viral replication monitored 72 h later by flow cytometry after staining with a JEV-specific antibody. Percentage of infected cells is indicated. (B) cDNA prepared from Neuro 2a cells stably transduced with shLuc- or shFvEJ- (left), or shLuc- or shFvEJ- injected mouse brains obtained 4 h (middle) or 24 h (right) after injection were subjected to RT-PCR to measure the induction of IFN-response genes. The PCR products were quantified by NIH Image J (version 1.32j) software. Normalized values obtained for the test samples were divided by that obtained with untreated Neuro 2a cells or untreated mouse brains to determine the changes in mRNA levels for each of the genes. (33 KB PDF).