WGS of Streptococcus porcinus SS-841, Streptococcus pseudoporcinus SS-607 and SS-662.

Whole genome sequencing of Streptococcus porcinus SS-841, S. pseudoporcinus SS-607 and SS-662 was performed as described below. Genomic DNA was isolated using the MasterPure Gram-positive DNA purification kit (Epicentre). Sequencing libraries were prepared using the NEBNext Ultra II DNA library prep kit (New England Biolabs). 250 ng DNA was sheared to 400 bp fragments in microTUBE screw-cap tubes in a M220 focused-ultrasonicator (Covaris). Following DNA library preparation, the library size was determined with a Bioanalyzer 2100 (Agilent), quantified using the Qubit dsDNA BR kit (Life Technologies), pooled in equal quantities, and analysed with the NEBNext library quant kit (New England Biolabs). The pooled library was subjected to 75 bp paired-end sequencing on the Illumina MiSeq platform using v3 chemistry.