Effect of KDM5 histone demethylase in 3T3-L1 differentiation

The KDM5 histone demethylases regulate histone methylation to modulate chromatin structure and gene expression. Using a KDM5 enzyme inhibitor (KDM5-C70) and transcriptome profiling by RNA-sequencing in 3T3-L1 cells at different states of differentiation, we determined that KDM5 activity influences the induction of genes associated with cell cycle regulation and mitochondrial function. Overall design: 3T3-L1 preadipocytes were treated with 10 µM KDM5-C70 (KDM5 inhibitor) or DMSO control at differentiation Day –2 (100% confluency). Cells were collected for RNA at differentiation Day 0, 1, 2, and 7.