rCRUX Generated Kelly Metazoan 16S Reference Database

rCRUX generated reference database using NCBI nt blast database downloaded in December 2022.

Primer Name:  Kelly Metazoan 16S
Gene:   16S
Length of Target:    114-160
get_seeds_local() minimum length:    80
get_seeds_local() maximum length:    195
blast_seeds() minimum length:    50
blast_seeds() maximum length:    168
max_to_blast:  100
Forward Sequence (5'-3'):   AGTTACYYTAGGGATAACAGCG
Reverse Sequence (5'-3'):    CCGGTCTGAACTCAGATCAYGT
Reference:    Kelly, R. P., O’Donnell, J. L., Lowell, N. C., Shelton, A. O., Samhouri, J. F., Hennessey, S. M., ... & Williams, G. D. (2016). Genetic signatures of ecological diversity along an urbanization gradient. PeerJ, 4, e2444. https://doi.org/10.7717/peerj.2444

We chose default rCRUX parameters for get_blast_seeds() of percent coverage of 70, percent identity of 70, evalue 3e+7, and max number of blast alignments = '100000000' and for blast_seeds() of coverage of 70, percent identity of 70, evalue 3e+7, rank of genus, and max number of blast alignments = '10000000'.