High-throughput capture of actively transcribed region-interacting sequences reveals an intricate promoter-centered regulatory network

1. FigS10A_CTCF_VSCTCF_IAA_WB: Western blot analysis of CTCF before and after IAA-induced degradation in K562 cells. 2. FigS1E_dotplot: Dot blot assay for different incubation time points of Large (Klenow) Fragment and dNTPs (with biotin-dCTP).1. FigS10A_CTCF_VSCTCF_IAA_WB：对K562细胞中经吲哚乙酸（IAA，Indole-3-acetic acid）诱导降解前后的CCCTC结合因子（CTCF）开展蛋白质免疫印迹（Western blot）分析。 2. FigS1E_dotplot：针对不同孵育时间下的Klenow大片段（Large (Klenow) Fragment）与脱氧核糖核苷三磷酸（dNTPs，含生物素标记的脱氧胞苷三磷酸（biotin-dCTP））所进行的斑点印迹实验（Dot blot assay）。