Kinetic parameters of NUDT16 towards ITP, XDP and ADP and comparison with the results from Iyama et al [14].

Km, kcat and kcat/Km were determined by fitting the experimental kinetic data to a Michaelis-Menten model in GraphPad Prism version 5.01 for Windows, GraphPad Software, San Diego California USA, www.graphpad.com. The values indicated by the columns labelled “Ref. 14” are issued from the work of Iyama et al [14]. Substrates indicated with an asterisk (*) (IDP and GDP) are those whose low Km values (according to Iyama et al [14]) are not suitable to have their kinetic parameters determined using malachite green assay.