Data showing positive regulation of Wnt/β-catenin signal transduction by FAM129B siRNA using transcriptional reporter assay, target gene expression, and apoptosis assay

Data for Figure 2b. Measured β-catenin dependent firefly: constitutive renilla ratios for HT1080, A375, and A2058 reporter cells treated with the indicated conditions. Data for Figure 2c: Quantitative RT-PCR of β-catenin dependent Axin2 mRNA and housekeeping actin mRNA for HT1080, A375, and A2058 cells treated with the indicated conditions. Data for Figure 3a. Measured β-catenin dependent firefly: constitutive renilla ratios for A375 reporter cells treated with a dose curve of WNT3A or a single dose of WNT3A. Data for Figure 3b. Measured NFκB dependent firefly: constitutive renilla ratios for A375 reporter cells treated with a dose curve of TNFalpha or a single dose of TNFα . Data for Figure 4b. Quantitation of DAPI and TUNEL positive nuclei from A375 cells treated with WNT3A, PLX4720, and the control, FAM129B, or CTNNB1 siRNA.

图2b数据：测定依赖于β-catenin的荧光素酶：HT1080、A375和A2058报告细胞的组成型Renilla比例，受指定期望条件处理。图2c数据：对HT1080、A375和A2058细胞中依赖于β-catenin的Axin2 mRNA和管家型肌动蛋白mRNA进行定量RT-PCR，受指定期望条件处理。图3a数据：测定依赖于β-catenin的荧光素酶：A375报告细胞在WNT3A剂量曲线或单个WNT3A剂量处理下的组成型Renilla比例。图3b数据：测定依赖于NFκB的荧光素酶：A375报告细胞在TNFalpha剂量曲线或单个TNFα剂量处理下的组成型Renilla比例。图4b数据：对A375细胞在WNT3A、PLX4720及对照组、FAM129B或CTNNB1小干扰RNA处理下DAPI和TUNEL阳性核的定量。