Assembly of mtDNA from wild lettuce species (L. saligna, L. serriola & L. virosa)

In this study, we used an Illumina MiSeq paired-end reads to build the mtDNA of 2 L. sativa accessions, 1 L. saligna accession, 3 L. serriola accessions & 3 of L. virosa accessions. Sequenced DNA was extracted from purified mitochondria isolated from etiolated seedlings on percoll gradient (±1200 seedlings ). Libraries were prepared with the Nextera XT Library kit, according to the manufacturer's recommendations (Illumina) using 100 ng of each DNA sample. Final libraries were quantified, checked on a Bioanalyzer 2100 (Agilent), and sequenced on an Illumina Miseq system (2 × 150 paired-end reads).