Gene expression profiles of RALD iPSCs cultured with or without bFGF

We investigated roles of KRAS on stemness maintenance in the context of induced pluripotent stem cells (iPSCs) using isogenic KRAS mutant (G13C/WT) and wild-type (WT/WT) iPSCs from the same RAS-associated autoimmune lymphoproliferative syndrome-like disease (RALD) patients. Microarray analysis was conducted to compare gene expression profiles of WT/WT and G13C/WT iPS cells cultured with or without bFGF. Expression of stemness markers including POU5F1 and NANOG, which is indicative of an undifferentiated state, was highly maintained in G13C/WT iPSCs, but not in WT/WT iPSCs, under bFGF depletion, indicating enhanced self-renewal of iPSCs by the oncogenic KRAS. To investigate the changes of undifferentiation and differentiation markers between KRAS mutant and wild-type iPSCs under the presence or absence of bFGF, the iPSCs were cultured with or without bFGF for 5 days. Two clones were used for each genotype, resulting in total 8 conditions.