Bruno1 regulates gene expression and splicing that is necessary for proper development of the indirect flight musculature in D. melanogaster.. Bruno1 regulates gene expression and splicing that is necessary for proper development of the indirect flight musculature in D. melanogaster.

Building on a previous study where we examined changes in gene expression and splicing after knockdown of Bruno 1 (Bru1, Aret) in indirect flight muscle, we have now generated a CRISPR allele bru1[M3] that results in a phenotypic null. Here we examine changes in gene expression and alternative splicing in bru1[M3] mutant IFM dissected from 1 d adult flies. Overall design: Analysis of differential gene expression and exon use between control (w[1118]) IFM and bru1 mutant (bru1[M3]) IFM from 1d adult.