Analysis on bacterial community structure of piggery wastes treatment by microbial nest technology

The V3-V4 hypervariable region of 16S rRNA gene was sequenced and analyzed by Illumina Miseq sequencing platform. Based on the richness, diversity, uniformity and coverage of microbial community in alpha diversity analysis, it is found that species richness, diversity, uniform distribution and coverage are high in high temperature period; There were significant differences among Pseudoxanthomonas, bacillus, Pantoea, Ochrobactrum, Streptomyces, Microbacterium and Enterobacter in the microbial nest at different periods. The dominant bacteria in high temperature period were proteus Bacillus of Firmicutes of Proteobacteria, Streptomyces and Pseudomonas of Actinobacteria, these bacteria are mainly organic matter and cellulose degrading bacteria; the relevant order of the impact of environmental factors on the bacterial community was moisture content > temperature > C/N > pH > EC > TN > TC > E4/E6 > NO3--N > NH4+-N, and the water content has the greatest impact on the bacterial community; At the same time, it was found that the functional abundance of denitrification in high temperature period is relatively high, indicating that there are a large number of denitrifying bacteria in the microbial nest, which convert nitrogen-containing compounds into nitrous oxide or discharge nitrogen, which may be one of the reasons for the decrease of total nitrogen in microbial nest.