TLR and Tck inhibition by IC is mediated via FcγRIIb and PI3K.

gmMφ were pre-incubated for 30 minutes with the FcγRIIb specific blocking antibody 2B6 (10 µg/ml) or an isotype control before stimulation with IC (50 µg/ml) and LPS (100 ng/ml) (A) or Tck (B). gmMφ were pre-incubated with Wortmannin (0.1 µM) or a Cox2 inhibitor (20 µM) for 1 hr before stimulation with IC and LPS (C) or Tck (D). 20 hour supernatants were collected to analyze TNFα levels. In the graphs showing TLR stimulation (A and C) the percentage of TNFα production is plotted with the LPS only stimulation set at 100%. In the Tck graphs (B and D) absolute values are shown. All graphs show the mean and SEM of at least 3 experiments. *P