Deamidated route: estimates of NMNAT and NADS physiological activities.

aSum of the three NMNAT isozyme activities individually calculated as in Table 2 but versus the NaMN substrate (Km values used are reported in ref [19]).bValues are calculated from the steady-state kinetic equation (3) shown in Methods, using the NADS activities measured at saturating substrates (Figure 3A) as Vmax values, the NaAD, ATP, and glutamine levels (nmol/g, Figure 4) as micromolar concentrations, and the Km values from [43].Deamidated route: estimates of NMNAT and NADS physiological activities.