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High resolution and allelic-specific annotation of preimplantation embryo transcriptome using long-read sequencing

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We used an combination of long-read and short-read sequencing data to explore the transcriptome of preimplantation embryo. We selected 7 stage of early embryo including sperm and oocyte from male DBA/2J and female C57/BL6J and sequenced using Pacbio and Illumina.

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