Whole genome expression profiling of Artemsinin resistant Plasmodium falciparum field isolates II

The objective of this study is to characterize gene expression signatures associated with in vivo artemisinin resistance phenotype and its transcriptional response to Artemisinin Combination Therapy (ACT) treatment . To achieve this goal, we employed microarray technology to establish the global gene expression profiles of isolates sampled from 680 patients prior to ACT treatment and 659 patients after the ACT treament. P. falciparum isolates were sampled from the whole blood of 680 patients from 6 countries and 13 sites across Southeast Asia (Ramu in Bangladesh; Thabeikkyin, Pyay, Pyin Oo Lwin and Ann in Myanmar; Phusing and Khun Han in Thailand; Pailin, Pursat, Ratanakiri and Preah Vihear in Cambodia; Sekong in Laos; Bin Phuoc in Vietnam) from 2015 to 2018. RNA was extracted and synthesis and amplification of cDNA were carried out to generate sufficient material for hybridizations against the RNA reference pool of 3D7 strain using a custom made microarray platform.