S-phase independent silencing establishment in Saccharomyces cerevisiae

As part of a study of establishment of silencing in Saccharomyces cerevisiae, we performed ChIP-seq on myc-tagged Sir4 in several conditions. Included in those conditions are wild-type cycling cells, cycling sir3? cells, and various experiments during which silencing establishment was controlled using the inducible SIR3-EBD allele. Silencing establishment experiments were performed in both wild-type and dot1? cells. Overall design: Sir4-13xMyc ChIP-seq, Sir3-EBD ChIP-seq and H3K79me3 ChIP-seq. For all key experiments, replicates were included.