RNA-Seq analysis to study the transcriptomic profiles of mice after 4-month wheel running exercise. RNA-Seq analysis to study the transcriptomic profiles of mice after 4-month wheel running exercise

To identify the mechanism underlying AdipoR1-mediated beneficial effect of exercise, we used RNA-Seq analysis to study the transcriptomic profiles of mice after 4-month wheel running exercise. Differential expression analysis was performed by comparing exercised mice to control mice, and exercised mice subjected to AdipoR1 shRNA versus exercised mice. Overall design: The transcriptional profiles of gastrocnemius muscle of exercised mice, control mice, and exercised mice subjected to AdipoR1 shRNA were generated by deep sequence, in quadruplicate, using Illumina Novaseq™.