Post-Transcriptional Regulation of Mouse Neurogenesis by Pumilio Proteins [RNA-Seq]

We employ mRNA-seq to investigate transcriptome of Pum1-Knockout, Pum2-Knockout and WT conditons In order to investigate whether Pum1 and Pum2 regulate their targets at their RNA levels, we used 1/10 of the samples from the Pum1 and Pum2 iCLIP experiments (four biological repeats of WT, P1KO, and P2KO neonatal brains) to extract total RNAs for RNA deep sequencing. And we also collected three Ndcko neonatal brains for RNA deep sequencing.本研究采用mRNA测序（mRNA-seq）技术，对Pum1基因敲除（Pum1-Knockout, P1KO）、Pum2基因敲除（Pum2-Knockout, P2KO）及野生型（Wild Type, WT）三种条件下的转录组进行分析。为探究Pum1与Pum2是否在RNA层面调控其靶标基因，我们取用Pum1和Pum2 iCLIP实验中1/10的样本（包含WT、P1KO及P2KO新生小鼠脑组织的4个生物学重复样本）提取总RNA，用于RNA深度测序。此外，我们还收集了3例Ndcko新生小鼠脑组织样本，开展RNA深度测序。