While high concentration FGF2 (50 ng/ml) suppressed BMP-2 mediated adipogenesis, low concentration FGF2 (0.4 ng/ml) did not further enhanced BMP-2 mediated adipogenesis.

Human ASCs were pre-conditioned in the growth medium (GM) with various concentrations of FGF2 or 150 ng/ml BMP-2 for 1 day, washed with PBS, treated in the differentiation medium (DM) for 6 days, and subjected to extraction of total RNA. Analysis of expression of aP2 gene was carried out using real-time PCR with cyclophilin as an internal control. Results are presented as means ± SD; n (numbers of experiments performed) = 3. (PDF)