Myeloperoxidase is required for ethanol-induced expression and release of Shh.
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A) Shh accumulation in kidneys of ethanol-fed mice depends on myeloperoxidase. Kidneys of wild-type BL6 or mpo-/- mice chronically ingesting ethanol or a control diet were excised, fixed and fluorescently stained for Shh, Gli1, or nuclei with DAPI. The resulting images were overlaid to generate yellow co-localized proteins as in Fig 3. B) MPO is required for Shh accumulation in urine. Urine of wild-type and mpo-/- mice collected at the end of the feeding trail was resolved and immunoblotted for urinary Shh as in Fig 5.
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2016-03-03
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