Replication Data for:CELL-REPORTS-D-23-04977R2

Figure 2A: BALB/c mice were transurethrally infected with WT, Δndk, or Δndk+ for 6 h p.i. The bladder tissues were analyzed for the amount of UpIII by western blotting
Figure 3A:Western blot analysis of the NDK protein levels in the culture supernatant and pellet of WT, Δndk, or Δndk+ that was cultured in logarithmic phase (OD600=0.6), and a periplasm protein DnaK was used as a loading control to eliminate the possibility that the presence of NDK in culture supernatant is from dead bacteria.
Figure 3C:5637 cells were infected with WT, Δndk, or Δndk+ for 4 h p.i. The cell lysates were analyzed for the amount of cleaved Caspase-1 p10 and pro-Caspase-1 by western blotting.
Figure 3D:The amount of full-length GSDMD and N-GSDMD in 5637 cells infected with WT, Δndk, or Δndk+, respectively, at 4 h p.i.
Figure 3E:The amount of full-length GSDMD and N-GSDMD in mouse bladders infected with WT, Δndk, or Δndk+, respectively, at 6 h p.i.
Figure 3F:Western blot analysis of the NDK protein levels in CFT073 infected 5637 cells supernatant and pellet.