The restriction modification systems in Clostridium carboxidivorans

One current roadblock in the development of tailored production strains from the wild type is the limited availability of genetic techniques for the modification of this organism. The Restriction Modification (RM) system of this species has been discussed as an explanation for its limited genetic accessibility. The genome C. carboxidivorans P7 (DSM 15243) harbors genes coding for 10 restriction enzymes (RE) and 8 methyltransferases (MT).