Development of a noninvasive genotyping-in-thousands (GTseq) panel for long-term conservation of western Great Lakes gray wolves (Canis lupus)

This file explains all of the variables in each of the datasets that accompany: 

Development of a noninvasive genotyping-in-thousands (GTseq) panel for long-term population conservation

Samuel D. Hervey, Bridgett M. vonHoldt, Mark C. Romanski, and Kristin E. Brzeski

HERVEY2025_SupplementaryMaterials.docx>>word document containing the supplementary information, figures and, tables referenced from the main text

HERVEY2025_Genotypes.xlsx>>excel spreadsheet containing all GTseq microhaplotypes and microsatellite genotypes to recreate all figures from the main text. Spreadsheet also contains the IDs and population IDs for RADseq data with connecting ID to GTseq data when comparing the two methods. See Summary tab within spreadsheet for more detail on specific fields of information.

HERVEY2025_WGL_GTseq_LocusSpecific_Indexing_NextSeqCustom_Oligos.xlsx>>excel spreadsheet with lists of locus specific primers for PCR1, indexing primers for PCR2, and custom primer for NextSeq 1000/2000 library preps.

flip_trim_sbfI_150821.pl>>custom perl script for retaining reads with Sbf1 cut site and demultiplexing library pools.

HERVEY2025_Figure5-RADseq>>VCF file of SNPs from RADseq data for recreating structure plot from figure 5 in the main text.

HERVEY2025_Figure6-RADseq>>VCF file of SNPs from RADseq data for recreating PIDsib calculation from main text and kinship analyses from CKMRsim.