Kinetics upon TCR-CD3 engagement.

tcontact: contact time of the initial T cell-bead contact. tcontact is taken as time reference t = 0.tpush: beginning time of the pushing phase (relative to tcontact = 0). tpush also marks the end of the latency phase.Δtpush: duration of the pushing phase, Δtpush = tpull−tpush.tCalcium: starting time of the time of the [Ca2+]i increase.tpull: beginning time of the pulling phase (relative to tcontact = 0).