26 By Biosemi

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1.Study introduction: First, participants underwent a series of laser stimulations of varying intensities. The experimenter determined the energy intensity corresponding to an average score of 7 points among the participants. Subsequently, each participant received a fixed-intensity laser stimulation approximately every 20 seconds, for a total of 16 trials, during which they provided pain ratings ranging from 0 to 10. A rating of 0 indicated no sensation, 4 denoted the onset of pain perception, 6 represented moderate pain, 8 indicated severe pain, and 10 signified intolerable pain. 2.Participant task information(description of the experiment): Participants received laser stimulation and used a computer mouse to click on the appropriate position on the screen, corresponding to a scale of 0 to 10. 3.Participant instructions(as exact as possible): Participants were instructed to concentrate on the laser stimuli, maintain open eyes, and fixate their gaze on a cross displayed on the screen before them. Following the presentation of each laser stimulus, there was a pause of 3¨C5 seconds. Subsequently, participants orally rated the intensity of pain within a time window of 3¨C5 seconds for each rating. The subsequent trial commenced randomly within 1¨C3 seconds after the ratings were provided. 4.References and links: Lu X, Thompson WF, Zhang L, Hu L. Music Reduces Pain Unpleasantness: Evidence from an EEG Study. J Pain Res. 2019 Dec 13;12:3331-3342. doi: 10.2147/JPR.S212080. PMID: 31853196; PMCID: PMC6916681.