Integration of network pharmacology, transcriptomics, and experimental verification to investigate the mechanism of action of cepharanthine hydrochloride against prostate cancer

Transcriptomic sequencing and differential expression analysis were used to identify the key target protein of CH and the key signaling pathways involved in its therapeutic effects against PCa. Overall design: Comparative gene expression profiling analysis of RNA-seq data for PC-3 cells(Cepharanthine hydrochloride 5µM) and control cell