Placental whole genome RNA sequencing dataset of monochorionic diamniotic twin pregnancies with and without selective fetal growth restriction

To compare placental transcriptomes between selective fetal growth restriction (sFGR) twins and normally grown twins (Control) in monochorionic diamniotic (MCDA) twin pregnancies, placentae from five sFGR and three control twin pairs were collected. Their placental transcriptomes were profiled by high throughput total whole genome RNA sequencing technology, which provides a global and deep examination of gene expression. The log2 ratio of the placental transcriptome expression levels of the smaller twin to that of the larger co-twin were calculated in each twin pair. Results in sFGR pregnancies were compared to that of the control pregnancies. Differentially expressed transcripts identified as the within-pair log expression ratio difference between sFGR and control groups were determined by Mann-Whitney U test. Based on these differentially expressed transcripts, biological pathways and upstream regulators were predicted using the Ingenuity Pathway Analysis (IPA) software. This dataset describes total 1429 differentially expressed placental transcripts, total 126 predicted biological pathways and 419 upstream regulators in sFGR. The data provide further insight in transcriptomic level to the pathomechansim of pregnancies complicated by sFGR.
The dataset supports the research article entitled “Monochorionic twins with selective fetal growth restriction: insight from placental whole transcriptome analysis”.

为比较选择性胎儿生长受限（sFGR）双胞胎与正常生长双胞胎（对照组）在单绒毛膜双羊膜囊（MCDA）双胎妊娠中的胎盘转录组，收集了五对sFGR和三对对照组双胞胎的胎盘样本。通过高通量全基因组RNA测序技术对这些胎盘的转录组进行了分析，该技术能够实现对基因表达的全局和深度考察。计算了每对双胞胎中较小双胞胎与较大同胎的胎盘转录组表达水平的log2比值。将sFGR妊娠的结果与对照组妊娠结果进行比较。通过Mann-Whitney U检验确定了sFGR组与对照组之间对内对数表达比值的差异所识别的差异表达转录本。基于这些差异表达转录本，利用Ingenuity Pathway Analysis（IPA）软件预测了生物学通路和上游调节因子。本数据集描述了sFGR中的1429个差异表达胎盘转录本、126条预测的生物学通路和419个上游调节因子。这些数据在转录组水平上进一步揭示了由sFGR引起的妊娠并发症的病理机制。该数据集支持题为“选择性胎儿生长受限的单绒毛膜双胞胎：来自胎盘全转录组分析的见解”的研究论文。