A high-throughput workflow for accurate exploitation of sex-specific markers: a case study on <i>Oplegnathus fasciatus</i>

The development of next-generation sequencing technology allowed the rapid exploitation of markers for genetic sex identification. Using male-female linear genomic information combined with Illumina survey and PacBio continuous long read (CLR) / circular consensus sequence (CCS) data, a database of large-segment (&gt;100 bp) insertion‒deletion genetic variants was constructed using a genome-wide variant site scanning method with bidirectional comparisons.

下一代测序（next-generation sequencing）技术的进步，使得遗传性别鉴定用的分子标记得以快速开发与应用。本研究结合Illumina测序数据与PacBio连续长读长（continuous long read, CLR）/环形共识序列（circular consensus sequence, CCS）数据，利用雌雄个体的线性基因组序列信息，采用全基因组变异位点双向比对扫描方法，构建了大片段（>100 bp）插入缺失遗传变异数据库。

搜集汇总

背景与挑战

背景概述

该数据集提供了一个高通量工作流程，用于准确开发性别特异性标记，以条石鲷（Oplegnathus fasciatus）为案例研究。数据集包含基于Illumina和PacBio测序数据构建的大片段插入-缺失变异数据库，支持遗传性别鉴定，适用于海洋鱼类性别鉴定方法的探索。