CircRNA expression profiling in cortical microglia isolated from 6-month-old male WT and APP/PS1 mice by magnetic-activated cell sorting (MACS)

To investigate the roles of circRNAs in the microglia of AD, we isolated cortical microglia from 6-month-old male wild-type (WT) and APP/PS1 mice by MACS using a CD11b antibod and conducted a circRNA microarray to profile the circRNA expression spectrum. A total of 13420 circRNAs were detected and 218 differentially expressed circRNAs (with a fold change＞1.5, P＜0.05) were found. 146 circRNAs were upregulated while 72 circRNAs were downregulated in cortical microglia of APP/PS1 mice compared with WT mice. CircRNA expression in cortical microglia isolated from 6-month-old male WT and APP/PS1 mice was measured.Three independent experiments were performed using different donors for each experiment.