NodulesData2025

Field pea plants were grown in winter (July to August) and spring (October to November) of 2023, outdoor under a rain-out shelter at the National University of Entre Ríos, Oro Verde, Argentina (31°49'50.0"S 60°31'14.6"W). Spring type, yellow-seed field pea cv Reussite was inoculated with Rhizobium leguminosarum biovar viceae (Rizobacter, Argentina) at 4 ml per kg seed and treated with systemic fungicide (Fludioxonil 2.5 g, Metalaxil-M 2.0 g and Tiabendazol 15 g) at 1 ml per kg seed. Starting N-NO3- content of the soil was 70.8 mg N kg⁻¹ soil in winter and 30.3 mg N kg⁻¹ soil in spring. Seeds were sown in 5-l pots filled with a mixture of local top-soil Acquic and Vertic Argiudoll of the Asociación Oro Verde serie (Plan Mapa de Suelos, 1998, Soil Survey Staff, 2014). Upper and lower limits of water holding capacity were assessed in a soil sample (Wilson et al., 2013). Water regimes were established targeting three levels of plant available water (PAW) in soil: 60%, 85%, and 100%; the premise is that 60 % PAW would cause drought stress, 100 % PAW may cause hypoxia stress but not anoxia, and 85 % PAW would allow for potential plant growth (Sadras and Milroy, 1996). At the V3 stage, treatments were initiated adjusting irrigation with a programmed controller (Rain Bird ESP-RZXe, USA). Irrigation frequencies were periodically monitored gravimetrically to keep actual soil moisture at ±5% of the target treatments. Water treatments were allocated to a completely randomized design with four replicates and each replicate consisted of two plants per pot.2023年冬季（7月至8月）与春季（10月至11月），试验于阿根廷恩特雷里奥斯国立大学Oro Verde校区（南纬31°49′50.0″，西经60°31′14.6″）的室外防雨棚（rain-out shelter）内开展。供试材料为春性黄籽田间豌豆（field pea）品种Reussite，播种前每千克种子接种4毫升豌豆根瘤菌（Rhizobium leguminosarum biovar viceae，阿根廷Rizobacter公司产品），并以每千克种子1毫升的用量施用内吸性复配杀菌剂，其组成为咯菌腈（Fludioxonil）2.5 g、精甲霜灵（Metalaxil-M）2.0 g与噻苯咪唑（Tiabendazol）15 g。试验土壤初始硝态氮（N-NO3⁻）含量分别为：冬季70.8 mg N·kg⁻¹土，春季30.3 mg N·kg⁻¹土。 试验采用5 L规格盆钵，装填取自Oro Verde协会系列的本地表土，该土壤为水成性变性黏化暗沃土（Acquic and Vertic Argiudoll），其分类依据来自1998年《土壤图图则》（Plan Mapa de Suelos, 1998）与美国土壤调查局（Soil Survey Staff, 2014）。通过土样测定得到该土壤的持水量上下限（Wilson et al., 2013）。 试验设置3个土壤植物有效含水量（Plant Available Water, PAW）梯度：60%、85%与100%，其中60% PAW将引发干旱胁迫，100% PAW可能导致低氧胁迫但不会引发厌氧胁迫，85% PAW则可支持植株潜在生长（Sadras and Milroy, 1996）。于植株V3生长阶段启动水分处理，通过可编程控制器（Rain Bird ESP-RZXe，美国）调节灌溉量。采用重量法定期监测灌溉频率，将实际土壤含水率维持在目标值的±5%范围内。水分处理采用完全随机设计，设置4次生物学重复，每盆种植2株植株。