Ultradeep-Pyrosequencing of PCR-Induced Mutations at Codon 65 in Plasmid Clones with Different KKK Nucleotide Patterns in Subtype B and Subtype C Genetic Contexts.

SDM, Site directed mutant; Amplicon subtype, subtype of RT codons 1–127; Underlined bases indicate bases changed by SDM; Reads at codon 65, average number of reads or sequence coverage at codons 64 to 66.
*Samples for which PfuUltra II Fusion was used in addition to Taq-Pwo polymerase. The % of K65R reads with PfuUltra II Fusion ranged from 0.02 to 0.05 for the three samples with the subtype C nucleotide patterns and 0 to 0.09 for the three samples with B nucleotide patterns.