Expression data from MCRedAL Prostate Tumors

Prostate tumor cells growth is interconected to androgen signaling. Indeed, the main line of treatment for advanced prostate cancer is focused on inhibiting androgen signaling by either surgical or pharmacological castration (or androgen-ablation, e.g. degarelix). We used microarrays to detail the global programme of gene expression following androgen-deprivation therapy (ADT) and identified distinct classes of up-regulated genes during this process that could contribute to the development of castration resistance. Prostate tumor cells were isolated for RNA extraction and hybridization on MTA1.0 Affymetrix microarrays. To obtain homogeneous populations of tumors at each treatment group (CS, castration sensitive; and pADT, Post-ADT), we isolated tumor cells from similar tumor size based on their positive expression of the mcherry fluorescent protein and negative expression of CD45, F4/80, and CD11b immune cell markers.