Identification of reported substrates of furin and thrombin using Query Sequence Set.

Furin and thrombin cleavage sequences from the natural substrates were extracted and a training set of different types, i.e. octa, tetra and tri peptides were created. The terapeptide sequence indicates P3P2P1P1�� and the tripeptide sequence indicates P3P2P1 residues. The ability of these query sequences to retrieve known/reported substrates was analyzed as described under methods.*Note that for the sake of clarity the number of proteins and not the number of cleavage sites are reported here.