Opportunistic diver-assisted eDNA sampling unpicks fine-scale ecological and conservation signals in tropical reef fishes

Description of the data and file structure This project contains raw reads data and ngs file for demultiplexing from eDNA samples collected from coral reefs in the Seychelles archipelago employing a versatile low-tech passive environmental DNA collector, the ‘metaprobe’, in association with the recreational activity of SCUBA divers. Raw reads files and ngsfilter files with sample barcodes are from a library prepared using the Elas02 12S barcode and sequenced with an iSeq Illumina sequencing platform. Files and variables File: Lib01_S1_L001_R1_001.fastq.gz Description: Forward FastQ file from the library sequenced with an Illumina iSeq 2x150 bp run File: Lib01_S1_L001_R2_001.fastq.gz Description: Reverse FastQ file from the library sequenced with an Illumina iSeq 2x150 bp run File: ngsSeychelles.txt Description: Primers and unique dual-tag combinations (forward and reverse) associated with each of the samples. The columns of the file correspond to: name of the project, samples, dual oligo-tags, forward primer and reverse primer. Variables name of the project name of the samples dual oligo-tags forward primer reverse primer File: MOTUTable_Seychelles.csv Description: Raw MOTU table where each row represents an OTU with its corresponding taxonomic assignment. Columns represent sampling sites, and the cell values indicate the number of sequence reads per OTU at each site. File: Seychelles_taxo_abund_dataset.csv Description: Final filtered dataset where only taxonomic assignments to species and/or genus level above 90% identity are kept. Each row represents an OTU with its corresponding taxonomic assignment, while columns represent sampling sites, and the cell values indicate the number of sequence reads per OTU at each site.