Maturation of Long Lived Plasma cells
收藏NIAID Data Ecosystem2026-05-01 收录
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To analyze global gene transcriptional changes and BCR clonal differences associated with long lived plasma cell specification, we sorted LLPCs and bulk PCs using Blimp1-ERT2-cre rosa26-LSLYFP expressing mice and analyzed them using bulk RNAsequencing on day 90 or day 5 after tamoxifen, YFP+ and YFP- CD138high B220- PCs were FACS-purified from BM, SPLEEN from young mice (age 6-8 for treatment) and middle-aged mice. (age 24 weeks at tamoxifen treatment), with 3-4 mice per group. Day 5 mice were aged 24 weeks before treatment
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2024-02-22
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